Cy5 glen research

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WebCy5/ Cyanine 5 are popular fluorescent dyes compatible with various detection techniques and standard filters. This class includes far-red fluorescence-emitting dyes with … WebCoupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 3.2 mg of the ROX NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt ...

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WebAll dyes except Cy5 were incorporated as the NHS ester via an aminohexyl linker in a separate reaction following oligonucleotide synthesis. For incorporation of Cy5, the phosphoramidite building block was used as received from Glen Research (Sterling, VA, USA). Water was deionized and filtered using a Milli-Q ® Synthesis A10 purification ... Webstable during deprotection, is widely used in the research.10 Here we report the study of Cy5/BHQ2, Cy5/BHQ3, sCy5/ ... and BHQ3-CPG were from Glen Research. Oligonucleotides maine\u0027s earned paid leave law

Cyanine 5.5 Phosphoramidite - Glen Research

WebTwo cyanine derivatives, Cyanine 3 and Cyanine 5, which differ in structure simply by the number of carbons in the conjugated poly- ene linkage, are joined by the closely related analogues, Cyanine 3.5 and Cyanine 5.5, and are available as phosphoramidites. Cyanine dyes are normally added once at the 5’-terminus and the MMT group should be ... WebClick here to view our reports! Sign up to receive technical advice and exclusive deals directly to your inbox. WebGlen Research offers the highest level of Quality Assurance for reagents for DNA and RNA synthesis - Sterling Quality and Performance. We now apply the Sterling criteria of quality and performance to all of Glen Research’s established products. common monomers and supports are available for the variety of synthesizers. Glen Research has developed and … maine\\u0027s first ship

Cy5.5-Poly-L-lysine-methoxypolyethylene glycol - PubMed

A device that operates within a self-assembled 3D DNA crystal

WebEXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefﬁcient of an oligo by either summing up the extinction coefﬁcients of the individual bases times their number of occurrences. WebGlen Research Technical Bulletin Extinctions3.html[7/16/19, 11:32:03 AM] EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. maine\u0027s golden road mapWebHowever, for dyes emitting in the far red, such as Cy5, the BHQ dyes were far superior – quenching the Cy5 with 96% efficiency, compared to 84% with Dabcyl. This may reflect the BHQ's ability to form stable, non-fluorescent complexes which can be a plus even in FRET probes. Indeed, recent work suggests that these non-fluorescent complexes ... maine\u0027s grand canyon

"WebGlen Research cyanine 5 cy5 phosphoramidite Cyanine 5 Cy5 Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 86/100, based on 1 … " - Cy5 glen research

Cy5 glen research

Spectral Characteristics of Fluorescent Dyes - Glen …

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WebAll solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on oligonucleotide synthesizers. View Quality Assurance. WebSpectral Characteristics of Fluorescent Dyes. Absorbance Maximum: Emission Maximum: Color: Fluorescein: 494nm: 525nm: Green: Tetrachloro-Fluorescein

WebGlen Report 34-11: Beyond the UV Region — DEACM-dG as a Versatile Tool for Light-Activatable (“Caged”) Oligonucleotides. Almost all major processes of life on the molecular and cellular scale are inherently three-dimensionally structured and time-dependent. Investigating these aspects requires tools that allow asking spatiotemporal questions.

Web13 rows · Minimal residual disease research; Drug discovery; Antibody Discovery; Drug … WebNHS Esters. An NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides, as well as peptides or proteins. NHS esters are especially useful in situations where the equivalent phosphoramidites exhibit instability to the conditions of oligonucleotide synthesis and/or deprotection.

WebGlen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the …

WebDescription. Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate ... maine\\u0027s first flagWebCyanine 3 and Cyanine 5 supports are also offered to allow simpler production of 3’ cyanine dye-labeled oligonucleotides. Deprotection of oligos containing Cyanine dyes may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. If there is a need to use ammonium hydroxide … maine\u0027s flyer binghamton nyWebAug 7, 2016 · We performed the comparison of dyes and quenchers (Cy5 vs. disulfo-Cy5 and BHQ2 vs. BHQ3) in several TaqMan probes with different stability of the secondary structure. Based on the studies of ... maine\u0027s flowerWebCy5.5-Conjugated anti-epidermal growth factor receptor monoclonal antibody C225. Cy5.5-Conjugated anti-human CD31 monoclonal antibody. Cy5.5-Conjugated glycol chitosan-5β-cholanic acid nanoparticles. Cy5.5 … maine\u0027s grocery store folsom laWebGlen Research cyanine 5 cy5 phosphoramidite Cyanine 5 Cy5 Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more maine\u0027s first ship bath maineWebGlen Research cyanine 5 cy5 phosphoramidite Cyanine 5 Cy5 Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 86/100, based on 1 … maine\\u0027s highest peakWebThe deprotection step is carried out at 65°C for 5 minutes. Deprotection can also be carried out at lower temperatures as shown in Table 2. In all cases, no base modification has been observed. Figure 1 illustrates the differences in RP HPLC between partially and fully deprotected oligos, DMT-off and DMT-on. Table 2. maine\\u0027s flower