Bmal1 chip-seq
WebJun 10, 2024 · Bmal1 is a key activator for cisplatin-induced renal hepatization Since Bmal1 facilitates cisplatin-induced renal injury, we wanted to identify the downstream effectors that mediate this... WebTo analyze the effect of PER2 on CLOCK–BMAL1-controlled gene expression, we first defined CLOCK–BMAL1-controlled genes as genes with transcription start sites within ±5 kb from BMAL1 binding sites. …
Bmal1 chip-seq
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WebOct 22, 2024 · BMAL1 regulates interferon-stimulated gene expression in lung epithelial cells To define whether circadian pathways regulate post-entry steps in the SARS-CoV-2 life cycle, we evaluated the effect of SR9009 on viral replication when added before or … Web1) Gained experience with and developed automated computational pipelines for the processing and analysis of Next Generation …
WebAug 20, 2024 · Deletion of Bmal1 has previously been demonstrated to lead to increased Hif1a mRNA in macrophages, and ChIP-Seq revealed BMAL1 binding to the regulatory regions of the Hif1α promoter . It is possible that we observe increased HIF-1α with deletion of Bmal1 through a lack of BMAL1-mediated suppression of Hif1α mRNA transcription, … WebMonoclonal Antibody for studying BMAL1. Cited in 55 publications. Validated for WB, IP, ChIP. Highly specific and rigorously validated in-house, BMAL1 (D2L7G) Rabbit Monoclonal Antibody (CST #14020) is ready to ship. ... For transcription factor or co-factor ChIP-seq, use at least 5 ng of ChIP-enriched DNA and amplification of the adaptor ...
WebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT12 of Bmal1 under the Misalignment protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. WebAug 20, 2024 · Our objective was to identify transcriptional targets of BMAL1 in undifferentiated (UNDIFF) and lactogen differentiated (DIFF) mammary epithelial cells (HC11) using ChIP-seq. Ensembl gene IDs with the nearest transcriptional start site to ChIP-seq peaks were explored as potential targets, and represented 846 protein coding …
WebJan 2024 - Oct 202410 months. San Diego, California, United States. • Experienced project leader utilizing molecular techniques and Next …
breckle diamond spring 100x200WebJun 15, 2013 · The circadian proteins CLOCK and BMAL1 heterodimerize and interact with E-box response elements in the promoters of target genes, which drive the positive transcription arm of the TTO loop. ... As mentioned above, genome-wide ChIP-seq analysis of the circadian proteins was recently performed in mouse liver samples over the 24-h … cotton wool spots hypertensive retinopathyWebJan 1, 2014 · To test the hypothesis that CLOCK:BMAL1 remodels chromatin, we first performed a genome-wide nucleosome analysis using MNase-seq (digestion of … breckle classic springWebimmunoprecipitation at the genome wide level (ChIP-Seq) in the mouse liver, kidney, and heart. Our data revealed that the majority of BMAL1 DNA binding is tissue-specific, and … cotton wool spots vs myelinated nerve fiberWeb- Team lead in projects to perform ChIP-seq… Show more - Bioinformatics consultation, collaboration, and support to principal investigators and … breckle diamond springWebMar 26, 2024 · To explore this possibility, we performed chromatin immuno-precipitation sequencing (ChIP-seq) experiments with specific antibodies against BMAL1 and MAX proteins. This analysis revealed a large number of genomic regions bound by MAX (around 13,000 peaks), while BMAL1 binding was limited to about 800 regions ( Figure 3 A; … cotton wool teddie sweaterWebFeb 23, 2024 · A line of mammary epithelial cells with the BMAL1 86gene knocked out (BMAL1-KO) using CRISPR-CAS was created to verify ChIP-seq findings, 87and study … breckle diamond spring 80x200